Ali, S., elsaudi, A., Saeed, A., Abushady, A. (2022). MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN BUFFALOES. Egyptian Journal of Animal Production, 59(1), 9-18. doi: 10.21608/ejap.2022.114631.1032
sarah Gamal Ali; alia Ahmed elsaudi; Ayman Moustafa Saeed; Asmaa Mohammed Abushady. "MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN BUFFALOES". Egyptian Journal of Animal Production, 59, 1, 2022, 9-18. doi: 10.21608/ejap.2022.114631.1032
Ali, S., elsaudi, A., Saeed, A., Abushady, A. (2022). 'MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN BUFFALOES', Egyptian Journal of Animal Production, 59(1), pp. 9-18. doi: 10.21608/ejap.2022.114631.1032
Ali, S., elsaudi, A., Saeed, A., Abushady, A. MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN BUFFALOES. Egyptian Journal of Animal Production, 2022; 59(1): 9-18. doi: 10.21608/ejap.2022.114631.1032
MOLECULAR CHARACTERIZATION OF SOME CANDIDATE GENES IN PURE EGYPTIAN BUFFALOES AND CROSSBRED OF ITALIAN BUFFALOES
1department of biotechnology, animal production research institute
2Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.
3Department of Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt.
4Biotechnology school, Nile University, Sheikh Zayed, Giza, Egypt. Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.
Abstract
The goal of this work was to describe the sequences of some candidate genes (IGF-I, IGF-I receptor, and Leptin) that are associated with economically important quantitative aspects in dairy buffalo, such as reproductive and productive attributes, as well as milk composition. Ninety-nine dairy buffaloes were used to compare the pure Egyptian buffalo (PE) with the Egyptian–Italian crossbred G1 (25.0%), G2 (50.0%), G3 (62.5%), G4 (75.0%), G5 (87.5%), and G6 (94.0%), respectively. All buffaloes investigated were genotyped BB, which means they were negative for the SnaBI at position 224^225 (TAC^GTA) of the IGF-I regulatory region, and they were genotyped AA-positive for the IGF-I receptor TaqI at position 47^48 (T^CGA). They also tested positive for the leptin gene's Alu1 restriction site yielding three products with genotype GT that was 55-, 118-, and 205-bp in length (AG^CT). Finally, the PE and Egyptian-Italian crossbred demonstrate monomorphism since the two Bubaline populations are closely related and the genes in question are maintained. More research is needed to learn more about Egyptian-Italian buffalo crossbreeds before national crossbreeding initiatives may be expanded.
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