Document Type : Original Article
Authors
1
department of biotechnology, animal production research institute
2
Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.
3
Department of Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt.
4
Biotechnology school, Nile University, Sheikh Zayed, Giza, Egypt. Department of Genetics, Faculty of Agriculture, Ain shams University, Soubra El- Khaymah, Cairo, Egypt.
Abstract
The goal of this work was to describe the sequences of some candidate genes (IGF-I, IGF-I receptor, and Leptin) that are associated with economically important quantitative aspects in dairy buffalo, such as reproductive and productive attributes, as well as milk composition. Ninety-nine dairy buffaloes were used to compare the pure Egyptian buffalo (PE) with the Egyptian–Italian crossbred G1 (25.0%), G2 (50.0%), G3 (62.5%), G4 (75.0%), G5 (87.5%), and G6 (94.0%), respectively. All buffaloes investigated were genotyped BB, which means they were negative for the SnaBI at position 224^225 (TAC^GTA) of the IGF-I regulatory region, and they were genotyped AA-positive for the IGF-I receptor TaqI at position 47^48 (T^CGA). They also tested positive for the leptin gene's Alu1 restriction site yielding three products with genotype GT that was 55-, 118-, and 205-bp in length (AG^CT). Finally, the PE and Egyptian-Italian crossbred demonstrate monomorphism since the two Bubaline populations are closely related and the genes in question are maintained. More research is needed to learn more about Egyptian-Italian buffalo crossbreeds before national crossbreeding initiatives may be expanded.
Keywords
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