SUPEROXIDE DISMUTASE MIMICS )TEMPOL) IMPROVES IN VITRO OOCYTES MATURATION IN EGYPTIAN BUFFALOES

Document Type : Original Article

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Animal Production Department, Faculty of Agriculture, Cairo Univerisity

Abstract

The purpose of this study was to examine how Tempol, an exogenous antioxidant, affected the in vitro maturation of Egyptian buffalo oocytes at the molecular level. Cumulus-oocyte complexes (COCs) were recovered from the ovaries of slaughtered animals. Cumulus-oocyte complexes were matured in a maturation medium supplemented with different concentrations of Tempol (0, 0.5, 1, and 2 μM)2-24 h and then nuclear maturation and cumulus cells expansion rates were evaluated. Profiled the expression of two candidate genes regulating metabolic activity and antioxidant status were analyzed in the matured oocytes using Real-Time PCR. Tempol increased the rate of buffalo COC expansion. The nuclear maturation rate was significantly improved in the 0.5 μM group compared to the 1 and 2 μM groups and the control group. A significant decline in the transcriptional abundance of CPT2 was observed with the increase in the Tempol concentration in the maturation medium. While, the expression level of the antioxidant-related gene showed a lower level of expression of different genes used in comparison with all experimental groups. In conclusion, the 0.5 μM concentration of Tempol may improve oocyte maturation and molecular competence

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